Luminaris Color HRM qPCR Master Mix

Catalog number: K1032BID

Thermo Scientific™  Related applications: Genotyping & Genomic Profiling | Real Time PCR (qPCR)

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Description

Thermo Scientific Luminaris Color HRM Master Mix is a convenient, ready-to-use master mix designed for the accurate detection of DNA sequence variations using High Resolution Melt (HRM) analysis method. The Master Mix complements Thermo Scientific PikoReal Real-Time System and software to provide a complete solution for HRM analysis.

The Luminaris Color HRM Master Mix contains Hot Start Taq DNA polymerase in an optimized buffer system with EvaGreen™ fluorescent dye for robust and reliable performance in PCR and HRM. The chemically modified Hot Start Taq DNA polymerase has ultra low residual activity until thermally activated ensuring PCR specificity and sensitivity. The DNA binding dye EvaGreen™ enables discrimination of even the most challenging sequence differences (such as Type IV SNP) without PCR inhibition and with no apparent sequence preference. The Luminaris Color HRM Master Mix is supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. Mixing both components in a reaction turns the solution green. This provides a visual aid when pipetting and decreases the risk of errors during reaction setup, especially when using white reaction vessels.

Highlights

• High performance on most real-time instruments with HRM capabilities
• Dual-color system for easy reaction setup
• Superior discrimination of all four SNP classes
• High sensitivity—accurate results from 30 copies of genomic DNA
• Exceptional stability of fully assembled reaction—for high-throughput applications
• Optimized for PikoReal Real-Time System and software

Applications

• SNP genotyping
• Mutation discovery (gene scanning)
• Heterozygosity screening
• Bacterial genotyping
• CpG methylation analysis

Includes
• Luminaris Color HRM Master Mix (2X) includes chemically modified Hot Start Taq DNA Polymerase, EvaGreen™ fluorescent dye, dNTPs and MgCl2 in an optimized PCR buffer with a blue dye.
• 40x Yellow Sample Buffer with a yellow dye.
• Nuclease-free water.

Related Products
Luminaris Color HRM qPCR Master Mix
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Genotyping Target: Epigenetic Targets, SNPs (Unknown or Numerous)
Product Line: Luminaris
Product Size: 1250 reactions
Technique: HRM (High Resolution Melting) Analysis, Mutation Scanning

Contents & storage

Contains:
• 10 x 1.25 mL Luminaris Color HRM Master Mix (2X) containing chemically modified Hot Start Taq DNA Polymerase, EvaGreen™ fluorescent dye, dNTPs and MgCl2 in an optimized PCR buffer with a blue dye; sufficient for 1250 x 20 µL reactions
• 3 x 1.25 mL 40X Yellow Sample Buffer
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.

Documents

Manuals & protocols

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