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Maxima Hot Start PCR Master Mix (2X)

Catalog number: K1052

Thermo Scientific™  Related applications: PCR

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Description

Thermo Scientific Maxima Hot Start PCR Master Mix (2X) is a ready-to-use solution containing chemically modified Maxima Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg2+, and dNTPs. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 4 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase. Highlights

• Convenient—Maxima Hot Start Taq DNA Polymerase in a ready-to-use mix
• Four minute activation time
• High PCR specificity and sensitivity
• Room temperature PCR set up

Applications

• Hot Start PCR
• Routine PCR
• High throughput PCR
• Multiplex PCR
• Genotyping
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Maxima Hot Start Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Product Line: Maxima
Product Size: 500 reactions

Contents & storage

Contains:
• 10 x 1.25 mL Maxima Hot Start PCR Master Mix (2X), which includes Maxima Hot Start Taq DNA polymerase, 2X hot start PCR buffer, 0.4 mM of each dNTP, 4 mM Mg2+
• 10 x 1.25 mL Nuclease-free water

Store at -20°C.

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