Maxima Hot Start Green PCR Master Mix (2X)

Catalog number: K1061

Thermo Scientific™  Related applications: PCR

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Thermo Scientific Maxima Hot Start Green PCR Master Mix (2X) is a ready-to-use solution containing chemically modified Maxima Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg2+, and dNTPs. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 4 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase. It is capable of amplification of up to 3 kb from genomic DNA.

The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.


• Convenient—Maxima Hot Start Taq DNA Polymerase in a ready-to-use mix
• Hot start PCR—reaction set-up at room temperature
• Four minute activation time
• Direct loading of PCR product on gels
• High specificity and sensitivity of PCR


• Hot Start PCR
• Routine PCR
• High throughput PCR
• Multiplex PCR
• Genotyping

Related Products
Maxima Hot Start Green PCR Master Mix (2X)
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Maxima Hot Start Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Product Size: 100 reactions

Contents & storage

• 2 x 1.25 mL Maxima Hot Start Green PCR Master Mix (2X), which includes:
— Maxima Hot Start Taq DNA polymerase,
— 2X hot start PCR buffer,
— 0.4 mM of each dNTP,
— 4 mM Mg2+,
— density reagent, and
— two dyes for direct loading on agarose gels.
• 2 x 1.25 mL Nuclease-free water

Store at -20°C.


Manuals & protocols

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