Thermo Scientific™

DreamTaq Green PCR Master Mix (2X)

Catalog number:  K1081

Related applications:

PCR

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Description

Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.

Highlights

• Direct loading of PCR product on gel
• High yields and high sensitivity of PCR
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Robust amplification with minimal optimization
• Incorporates modified nucleotides, but does not incorporate dUTP

Applications

• Routine PCR amplification of DNA fragments up to 6 kb
• Genotyping
• High throughput PCR
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Hot Start: No
Product Line: DreamTaq
Reaction Format: SuperMix or Master Mix
Reaction Speed: Standard
Polymerase: DreamTaq DNA Polymerase
Overhang: 3'-A

Contents & storage

Contains:
• 4 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20°C.