Thermo Scientific™

LyticBLAzer™-FRET B/G Kit, 10X

Catalog number: K1150
Thermo Scientific™

LyticBLAzer™-FRET B/G Kit, 10X

Catalog number: K1150
Catalog Number
K1150
Unit Size
15 ml
Price (USD)
1,278.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
375 mL
Price (USD)
31,850.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K115015 ml
1,278.00
Your Price:
***
K1151375 mL
31,850.00
Your Price:
***
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LyticBLAzer™ -FRET B/G Substrate Kits provides a ratiometric, end-point method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -FRET B/G Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ on top-read or bottom-read instrumentation. LyticBLAzer™ Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.

LyticBLAzer™ FRET B/G Substrate Kit Advantages:

• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation
• Endpoint analysis and stable fluorescence yield longer read times

LyticBLAzer™ -BODIPY™ FL Substrate Kit:
LyticBLAzer™ -BODIPY™ FL Substrate Kits provides an end-point, non-ratiometric method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -BODIPY™ FL Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ -BODIPY™ FL Assays on top-read or bottom-read instrumentation. LyticBLAzer™ -BODIPY™ FL Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.

LyticBLAzer™ -BODIPY™ FL Substrate Kit Advantages:
• BODIPY™ fluorophore excitation avoids fluorescence interference caused by sample contaminants such as dust, lint, etc.
• Standard excitation and emission wavelengths are universally adaptable to all fluorescence instrumentation.
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation.
• Endpoint analysis and stable fluorescence yield longer read times

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)
Fluorometer, Microplate Reader
Detection Method
Fluorescent
Product Line
LyticBLAzer™
Substrate
LyticBLAzer™-FRET B/G
Substrate Properties
Chemical Substrate
Substrate Type
Beta-Lactamase Substrate
Product Type
Kits for Analysis of Beta-Lactamase Activity
Quantity
15 mL
Includes
10 Kits
Substrate Properties
Chemical Substrate
Substrate
LyticBLAzer™-FRET B/G

Contents & Storage

LyticBLAzer™ homogenous kits: LyticBLAzer™-FRET B/G Homogenous Loading Kits include LyticBLAzer™_h-FRET B/G Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-FRET B/G Substrate (provided lyophilized) should be stored at -20°C, and protected from light. LyticBLAzer™_h-FRET B/G Substrate should also be dessicated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22-25°C, protected from direct light.

The 150 ml kit is sufficient for 3,750 x 40 µl standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 µl standard protocol well assays. Bulk quantities of loading kits are available upon request.

LyticBLAzer™ standard kits: LyticBLAzer™-FRET B/G Loading Kits include LyticBLAzer™-FRET B/G substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-FRET B/G substrate is provided lyophilized and should be stored at -20“C, dessicated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22-25°C, protected from direct light.

The 150 ml kit is sufficient for 3,750 x 40 µl standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 µl standard protocol well assays. Bulk quantities of loading kits are available upon request.

LyticBLAzer™_h-BODIPY™ FL Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-BODIPY™ FL Substrate (provided lyophilized) should be stored at -20“C, and protected from light. LyticBLAzer™_h-BODIPY™ FL Substrate should also be
dessicated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22-25°C, protected from direct light.

LyticBLAzer™-BODIPY™ FL Loading Kits include LyticBLAzer™-BODIPY™ FL substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-BODIPY™ FL substrate is provided lyophilized and should be stored at -20°C, dessicated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22-25°C, protected from direct light.

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