GeneBLAzer® P2RY6 CHO-K1 DA Assay Kit

Catalog number: K1327

 Related applications: Cellular Imaging | Target & Lead Identification & Validation

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Description

This cell line is available as dividing cells by requesting a quote for K1253 from dividingcells@invitrogen.com

GeneBLAzer® P2RY6 CHO-K1 DA (Division Arrested) cells and P2RY6-NFAT-bla CHO-K1 cells contain the human purinergic receptor P2, G protein-coupled, 6 (P2RY6) receptor stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. #K1078) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both P2RY6 CHO-K1 DA cells and P2RY6-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Uridine 5’-diphosphate (UDP); (Figure 1). In addition, P2RY6-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

P2RY6 has been found in a variety of rat tissue such as; placenta, spleen, thymus, lung, stomach, intestine, mesentery, heart, and aorta. P2RY6 is activated by UDP, which functions as a full agonist. P2RY6 is activated either weakly or not at all by additional extracellular nucleotides such as UTP, ATP, and ADP. P2RY6 has been implicated to be involved in ion transport, vasodilatation, and contraction of smooth muscle.


Storage:

GeneBLAzer® P2RY6 CHO-K1 DA Cells and P2RY6-NFAT-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer® P2RY6 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•P2RY6 CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer® P2RY6 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•P2RY6 CHO-K1 DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer® P2RY6-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•P2RY6-NFAT-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Gene ID: 5031
Reporter: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Cell State: Division-Arrested Cells
Gene Symbol: P2RY6
Product Size: 1 plate
Accession Number: NM_004154.3
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Documents

Manuals & protocols