Thermo Scientific™

GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit

Catalog number:  K1329

Related applications:

Cellular Imaging

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Description

GeneBLAzer™ AVPR2 CHO-K1 DA (Division Arrested) cells and AVPR2-CRE-bla CHO-K1 cells contain the human Arginine Vasopressin 2 (AVPR2) receptor stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate) or a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both AVPR2 CHO-K1 DA cells and AVPR2-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of dDAVP (Figure 1). In addition, AVPR2-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Agonists and antagonists of the AVPR2 receptors are in various stages of clinical trials for the treatment of hypertension and as diuretics.


Storage:

GeneBLAzer™ AVPR2 CHO-K1 DA Cells and AVPR2-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•AVPR2 CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer™ AVPR2 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR2 CHO-K1 DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer™ AVPR2-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR2-CRE-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_000054
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene ID (Entrez): 554
Gene Symbol: AVPR2
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: AVPR2