Thermo Scientific™

GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit

Catalog number: K1329
Thermo Scientific™

GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit

Catalog number: K1329
Catalog Number
K1329
Unit Size
1 plate
Price (USD)
902.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K13291 plate
902.00
Your Price:
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GeneBLAzer™ AVPR2 CHO-K1 DA (Division Arrested) cells and AVPR2-CRE-bla CHO-K1 cells contain the human Arginine Vasopressin 2 (AVPR2) receptor stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate) or a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both AVPR2 CHO-K1 DA cells and AVPR2-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of dDAVP (Figure 1). In addition, AVPR2-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Agonists and antagonists of the AVPR2 receptors are in various stages of clinical trials for the treatment of hypertension and as diuretics.


Storage:

GeneBLAzer™ AVPR2 CHO-K1 DA Cells and AVPR2-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•AVPR2 CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer™ AVPR2 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR2 CHO-K1 DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer™ AVPR2-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR2-CRE-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_000054
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene ID (Entrez)
554
Gene Symbol
AVPR2
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
AVPR2
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs

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Citations & References