This cell line is available as dividing cells by requesting a quote for K1189 from dividingcells@invitrogen.com

GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA (Division Arrested) cells and FPRL-1-Gα15-NFAT-bla CHO-K1 cells contain the human Formyl Peptide-like 1 receptor (FPRL-1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cells (Cat. no. K1213) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element, in addition to the promiscuous G-protein, Gα15. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both FPRL-1-Gα15 CHO-K1 DA cells and FPRL-1-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC₅₀ concentrations of WKYMV(M); (Figure 1). In addition, FPRL-1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

The FPRL-1 receptor has been implicated in a variety of inflammation and neurological disorders, including Alzheimer’s disease.


Storage:

GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA Cells and FPRL-1-Gα15-NFAT-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•FPRL-1-Gα15 CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70μg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•FPRL-1-Gα15 CHO-K1 DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer™ FPRL-1-Gα15-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•FPRL-1-Gα15-NFAT-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis