GeneBLAzer® M1 CHO-K1 DA Assay Kit

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Description

This cell line is available as dividing cells by requesting a quote for K1230 from dividingcells@invitrogen.com

GeneBLAzer® M1 CHO-K1 DA(Division Arrested) cells and M1-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 1 receptor (M1) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no.K1078) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both M1 CHO-K1 DA cells and M1-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of carbachol, (Figure 1). In addition, M1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

The Musacarinic 1(M1) Receptor is localized principally in the cortical and hippocampal regions of the brain and has been implicated in various neurological conditions including depression, anxiety, and sleep disorders.


Storage:

GeneBLAzer® M1 CHO-K1 DA Cells and M3-NFAT-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer® M1 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•M1 CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer® M1 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol)..
Includes:
•M1 CHO-K1 DA cells
•1 protocol
•Certificate of Analysis

GeneBLAzer® M1-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•M1-NFAT-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Symbol: M1
Accession Number: NM_000738
Cell Line: CHO-K1
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene ID: 1128
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Product Size: 1 plate
Reporter: BLA (Beta-Lactamase)
System: GeneBLAzer™

Documents

Manuals & protocols