GeneBLAzer® PTGDR CHO-K1 DA Assay Kit

Catalog number: K1373

 Related applications: Cellular Imaging | Target & Lead Identification & Validation

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Description

This cell line is available as dividing cells by requesting a quote for K1241 from dividingcells@invitrogen.com

GeneBLAzer® PTGDR CHO-K1 DA (Division Arrested) cells and PTGDR-CRE-bla CHO-K1 cells contain the human Prostanoid (PTGDR) receptor stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both PTGDR CHO-K1 DA cells and PTGDR-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Prostaglandin D2 (Figure 1). In addition, PTGDR-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

The Adenosine A2A Receptor (PTGDR) has been shown to have many roles throughout the body. PTGDR is involved in pain and inflammation regulation, inhibition of platelet aggregation, blood pressure regulation and contributes to ischemic brain damage. Selective antagonists of the PTGDR pathway are being tested as adjuvants to dopaminergic drugs to treat Parkinson’s disease and schizophrenia.


Storage:

GeneBLAzer® PTGDR CHO-K1 DA Cells and PTGDR-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer® PTGDR CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•PTGDR CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer® PTGDR CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•PTGDR CHO-K1 DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer® PTGDR-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•PTGDR-CRE-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Gene ID: 5729
Reporter: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Cell State: Division-Arrested Cells
Gene Symbol: PTGDR
Product Size: 1 plate
Accession Number: NM_000953.2
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Documents

Manuals & protocols