Thermo Scientific™

GeneBLAzer™ RAR alpha DA Assay Kit

Catalog number: K1387
Thermo Scientific™

GeneBLAzer™ RAR alpha DA Assay Kit

Catalog number: K1387
Catalog Number
K1387
Unit Size
1 plate
Price (USD)
1,136.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K13871 plate
1,136.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
The GeneBLAzer®RAR alpha DA (Division Arrested) and RAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor alpha fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK293T cell line. GeneBLAzer®UAS-bla HEK 293T cells (catalog#K1104) stably express a beta-lactamase reporter gene under the transcriptional control of a 7x Upstream Activator Sequence (UAS). Transcription from the 7xUAS is activated by the binding of the GAL4 transcription factor DNA-binding-domain (DBD). The GAL4-DBD is expressed as a fusion protein with the ligand binding domain (LBD) of RAR alpha. When an agonist binds to the LBD of the GAL4(DBD)-RAR alpha(LBD) fusion protein it translocates to the nucleus where it binds to the 7x UAS inducing transcription of beta-lactamase. Division Arrested (DA) cells are available in an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate). DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR alpha DA cells and RAR alpha-UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, substrate loading time and have been validated for Z' and EC50 concentrations of all-trans retinoic acid. Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
Tetrac, T4
Antagonists
1-850
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene Symbol
RARα
Primary Agonist
T3 Hormone
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
RARA, RAR alpha, NR1B1
Cell Line
293T (HEK)
System Type
GeneBLAzer™
Druggable Target
Nuclear Receptors

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains sufficient division-arrested cells and substrate to assay one 384-well plate includes:RAR alpha HEK 293T DA cells (K1387A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Figures

Frequently asked questions (FAQs)

Citations & References