GeneBLAzer™ ER Alpha DA Assay Kit

Catalog number: K1393

GeneBLAzer™ ER Alpha DA Assay Kit

Catalog number: K1393
Catalog Number
K1393
Unit Size
1 plate
Price (USD)
1,140.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K13931 plate
1,140.00
Your Price:
***
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GeneBLAzer®ER alpha DA(Division Arrested) cells and ER alpha-UAS-bla GripTite™ cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla GripTite™ cell line. GeneBLAzer®UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER alpha DA cells and ER alpha-UAS-bla GripTite™cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
Betamethasone, Cortisol, Budesonide
Antagonists
Milfepristone, Cortisone, Progesterone
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene ID (Entrez)
2099
Gene Symbol
ERα
Primary Agonist
Dexamethasone
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
ER alpha, ESR1, ERA, NR3A1
Cell Line
293T (HEK)
System Type
GeneBLAzer™
Druggable Target
Nuclear Receptors

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER alpha GripTite™ DA cells (K1391A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg

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