GeneBLAzer™ FXR DA Assay Kit

Catalog number:  K1397

Related applications:

Cell Analysis

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Description

GeneBLAzer® FXR DA (Division Arrested) cells and FXR-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human farnesoid X receptor (FXR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer® UAS-bla HEK 293T cell line. GeneBLAzer® UASbla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-FXR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both FXR DA cells and FXR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of chenodeoxycholic acid (CDCA). In addition, FXR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists: GW 0742, Retinoic Acid, GW 7647, Bezafibrate
Antagonists: MK886
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: 293T (HEK)
Cell State: Division-Arrested Cells
Druggable Target: Nuclear Receptors
Gene Symbol: FXR
Primary Agonist: L-165, 041
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: FXR, NR1H4
Detection Method: Fluorescent

Contents & storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:FXR HEK 293T DA cells (K1397A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg