Thermo Scientific™

GeneBLAzer™ RXR beta DA Assay Kit

Catalog number:  K1405

Related applications:

Cellular Imaging

Back to top


GeneBLAzer®RXR beta DA (Division Arrested) cells and RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RXR beta DA cells and RXR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of 9-cis retinoic acid (9-cis-RA). In addition, RXR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
For Research Use Only. Not for use in diagnostic procedures.


Alternate Agonists: Progesterone
Antagonists: RU 486
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: 293T (HEK)
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Gene Symbol: RXRβ
Primary Agonist: R5020
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: RXR beta, NR2B2, RXRB

Contents & storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:RXR beta HEK 293T DA cells (K1405A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg