Thermo Scientific™

GeneBLAzer™ RAR-beta DA Assay Kit

Catalog number: K1407
Thermo Scientific™

GeneBLAzer™ RAR-beta DA Assay Kit

Catalog number: K1407
Catalog Number
K1407
Unit Size
1 plate
Price (USD)
1,136.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K14071 plate
1,136.00
Your Price:
***
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GeneBLAzer®RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Antagonists
none
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene Symbol
RARβ
Primary Agonist
9-cis-retinoic acid
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
RAR beta, NR1B2, RARB
Cell Line
293T (HEK)
System Type
GeneBLAzer™
Druggable Target
Nuclear Receptors

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:RAR beta HEK 293T DA cells (K1407A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg

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