Thermo Scientific™

GeneBLAzer™ RXR alpha DA Assay Kit

Catalog number:  K1411

Related applications:

Cellular Imaging

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Description

GeneBLAzer®RXR alpha DA(Division Arrested) cells and RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UASbla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of alpha-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RXR alpha DA cells and RXR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of 9-cis-retinoic acid. In addition, RXR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists: Dexamethasone, Progesterone, Cortisol
Antagonists: Spironolactone
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: 293T (HEK)
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Gene Symbol: RXRα
Primary Agonist: Aldosterone
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: RXRA, RXR alpha, NR2B1

Contents & storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:RXR alpha HEK 293T DA cells (K1411A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg