GeneBLAzer® PPAR gamma DA Assay Kit

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GeneBLAzer®PPAR gamma DA (Division Arrested) cells and PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293H cell line. GeneBLAzer®UAS-bla HEK 293H cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both PPAR gamma DA cells and PPAR gamma-UAS-bla HEK 293H cells are functionally validated for Z' and EC50 concentrations of Rosiglitazone. In addition, PPAR gamma-UAS-bla HEK 293H cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
For Research Use Only. Not for use in diagnostic procedures.


System: GeneBLAzer®
Gene ID: 5468
Reporter: BLA (Beta-Lactamase)
Cell Line: 293H
Cell State: Division-Arrested Cells
Gene Symbol: PPARγ
Product Size: 1 plate
Primary Agonist: Calcitriol
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors

Contents & storage

Shipping: Dry Ice Storage: -80°C/Liquid Nitrogen Sufficient division-arrested cells and substrate to assay one 384-well plate. Includes: PPAR gamma 293H DA cells (K1419A) LiveBLAzer™-FRET B/G Loading Kit, 70 μg


Manuals & protocols