Thermo Scientific™

GeneBLAzer™ ERR Alpha DA Assay Kit

Catalog number:  K1421

Related applications:

Cellular Imaging

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Description

GeneBLAzer™ERR alpha DA(Division Arrested) cells and ERR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Estrogen related receptor alpha (ERR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ERR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ERR alpha DA cells and ERR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of XCT790, an inverse agonist. In addition, ERR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists: L165, 041, GW 1929, GW 7647, GW 0742
Antagonists: T0070907, GW 9662, GW 6471
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: 293 T (HEK)
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Gene ID (Entrez): 2101
Gene Symbol: ERRα
Primary Agonist: Rosiglitazone
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: ESRRA, NR3B1, ERR1, ERR alpha

Contents & storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 µg