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Thermo Scientific™

GeneBLAzer™ CALCRL:RAMP1-CRE-bla FreeStyle™ 293F Cells

Catalog number:  K1437

Related applications:

Pharma & Biopharma

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Description

GeneBLAzer® CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells contain the human Calcitonin Gene Related Peptide Receptor (CALCRL:RAMP1), (Accession # NM_005795.3 NM_005855.2) stably integrated into the CellSensor® CRE-bla Freestyle™ 293F cell line. CellSensor® CRE-bla Freestyle™ 293F cells (Cat # K1130) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE).

CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells are functionally validated for Z'-factor and EC50 concentrations of Alpha-CGRP. In addition, CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_005855.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: FreeStyle™ 293-F Cells
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: CALCRL:RAMP1
Population Status: Single Cell Clone Origin
Product Line: Freestyle™, GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: CALCRL:RAMP1

Contents & storage

(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer® CALCRL:RAMP1-CRE-bla Freestyle™ 293F cells
• GeneBLAzer® CALCRL:RAMP1-CRE-bla Freestyle™ 293F Assay Protocol
• Certificate of Analysis

The GeneBLAzer® CALCRL:RAMP1-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.