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The GeneBLAzer® CALCRL:RAMP3-CRE-bla FreeStyle™ 293F cells contain the human Amylin Receptor 2 (CALCRL:RAMP3), (Accession # NM_005795.3 NM_005856.1) stably integrated into the CellSensor® CRE-bla Freestyle™ 293F cell line. CellSensor® CRE-bla Freestyle™ 293F cells (Cat # K1130) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE).
CALCRL:RAMP3-CRE-bla FreeStyle™ 293F cells are functionally validated for Z'-factor and EC50 concentrations of Adrenomedullin(1-52). In addition, CALCRL:RAMP3-CRE-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Cell-based beta lactamase reporter gene
Single Cell Clone Origin
FreeStyle™ 293-F Cells
Contents & Storage
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells • GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F Assay Protocol • Certificate of Analysis The GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.