GeneBLAzer® Gα15-NFAT CHO-K1 DA Assay Kit

Catalog number: K1453

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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The GeneBLAzer® Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor® NFAT-bla CHO-K1 (Cat #K1078) cell line. NFAT-bla CHO-K1 cells contain a beta-lactamase reporter gene under control of an NFAT response element stably integrated into CHO-K1 cells. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.
For Research Use Only. Not for use in diagnostic procedures.


System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Cell State: Division-Arrested Cells
Gene Symbol: Gα15
Product Size: 1 plate
Detection Method: Fluorescent
Druggable Target: GPCRs

Contents & storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
o GeneBLAzer® G?15-NFAT CHO-K1 DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o GeneBLAzer® G?15-NFAT CHO-K1 Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The GeneBLAzer® G?15-NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.


Manuals & protocols