CellSensor® CRE HEK 293T DA Assay Kit

Catalog number: K1457

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The CellSensor® CRE HEK 293T DA (Division Arrested) cells contains a beta-lactamase (bla) reporter gene under control of a cAMP response element (CRE) stably integrated into HEK293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the CRE pathway by forskolin. The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of forskolin. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can be used in other assays where a sensitive readout to intracellular changes to cAMP is needed.
For Research Use Only. Not for use in diagnostic procedures.


Cell State: Division-Arrested Cells
Druggable Target: GPCRs, Signaling Pathway
Product Line: CellSensor®
Product Size: 1 plate
System: CellSensor™

Contents & storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
o CellSensor® CRE HEK293T DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o CellSensor® CRE HEK293T CHO-K1 Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The CellSensor® CRE HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.


Manuals & protocols