β-Gal Staining Kit

Catalog number:  K146501

 Related applications:

Protein Assays and Analysis

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The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of X-gal, producing a blue color that can be easily visualized under a microscope.
For Research Use Only. Not for use in diagnostic procedures.


Assay Type: β-Gal (lacZ) Assay
Detection Method: Colorimetric
Product Size: 1 kit
Substrate: X-Gal
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase

Contents & storage

The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 µg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols