β-Gal Staining Kit

Catalog number: K146501

Invitrogen™  Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of X-gal, producing a blue color that can be easily visualized under a microscope.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: β-Gal (lacZ) Assay
Detection Method: Colorimetric
Product Size: 1 kit
Substrate: X-Gal
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase

Contents & storage

The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 µg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols

Recommended products