The GeneBLAzer™ ADRA1A-NFAT-bla CHO-K1 cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession # NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element.
Both ADRA1A-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_000680
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
Gene Symbol
ADRA1A
Population Status
Pool of Stable Cells
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
ADRA1A
Type
CHO-K1 Cells
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs
Contents & Storage
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o GeneBLAzer™ ADRA1A -NFAT-bla CHO-K1 cells o GeneBLAzer™ ADRA1A -NFAT-bla CHO-K1 Assay Protocol o Certificate of Analysis The GeneBLAzer™ ADRA1A -NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.