The GeneBLAzer® ADRB3 CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Beta 3 Receptor (ADRB3) (Accession # NM_000025.1) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the Cyclic AMP Response Element (CRE). The ADRB3 CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Isoproterenol. In addition, ADRB3-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.
Cell-based beta lactamase reporter gene
Single Cell Clone Origin
Contents & Storage
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: o GeneBLAzer™ ADRB3 CHO-K1 DA cells o LiveBLAzer™-FRET B/G Loading Kit, 70µg o Solution D o GeneBLAzer™ ADRB3 CHO-K1 Assay Protocol o LiveBLAzer™-FRET B/G Loading Protocol o Certificate of Analysis The GeneBLAzer™ ADRB3 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.