Thermo Scientific™

GeneBLAzer™ ADRB3 CHO-K1 DA Assay Kit

Catalog number: K1473
Thermo Scientific™

GeneBLAzer™ ADRB3 CHO-K1 DA Assay Kit

Catalog number: K1473
Catalog Number
K1473
Unit Size
1 plate
Price (USD)
902.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K14731 plate
902.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
The GeneBLAzer® ADRB3 CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Beta 3 Receptor (ADRB3) (Accession # NM_000025.1) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the Cyclic AMP Response Element (CRE).
The ADRB3 CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Isoproterenol. In addition, ADRB3-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_000025.1
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene Symbol
ADRB3
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
ADRB3
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o GeneBLAzer™ ADRB3 CHO-K1 DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o GeneBLAzer™ ADRB3 CHO-K1 Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The GeneBLAzer™ ADRB3 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Figures

Frequently asked questions (FAQs)

Citations & References