CellSensor™ T-REx™ NICD CSL-bla HeLa Cell Line
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Thermo Scientific™

CellSensor™ T-REx™ NICD CSL-bla HeLa Cell Line

The CellSensor™ T-REx™ NICD CSL-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with aRead more
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Catalog number K1487
Price (USD)/ Each
10,930.00
-
Add to cart
Price (USD)/ Each
10,930.00
Add to cart
CellSensor™ T-REx™ NICD CSL-bla HeLa Cell Line
Catalog numberK1487
Price (USD)/ Each
10,930.00
-
Add to cart

The CellSensor™ T-REx™ NICD CSL-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a notchresponse element driving beta-lactamase reporter gene expression (CSL-bla) along with tetracycline repressor and tetracycline (or the tetracycline analog, doxycycline)-inducible NICD (notch intracellular domain) constructs. This cell line is a clonal population isolated by flow cytometry. Addition of doxycycline to these cells allows for regulated NICD transcription factor expression and subsequent beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of parameters, including cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of doxycycline. Additional testing data using RNAi and alternate stimuli are also available.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay EntryCell-based beta lactamase reporter gene
Cell StateDividing Cells
Detection MethodFluorescence
Product LineCellSensor™, T-REx™
Quantity1 Vial
Target EntryNICD, Notch pathway
Cell LineHeLa
System TypeCellSensor™
Druggable TargetSignaling Pathway
Unit SizeEach
Contents & Storage
CellSensor™ T-REx™ NICD CSL-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.