During checkout, this product may be reported as "Out of Stock" with an Estimated Availability date. The Actual Availability date for this product may be earlier. Send inquiries regarding Actual Availability date, including the catalog number, to discoverysciences@lifetech.com.
Thermo Scientific™

LanthaScreen™ STAT3 GripTite™ Cell Line

Catalog number: K1488
Thermo Scientific™

LanthaScreen™ STAT3 GripTite™ Cell Line

Catalog number: K1488
Catalog Number
K1488
Unit Size
1 vial
Price (USD)
8,060.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K14881 vial
8,060.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
The JAK/STAT3 signaling pathway is known to be activated by cytokines such as IL-6. In this pathway, binding of IL-6 to its cell-surface receptors results in the activation of JAKs, which in turn phosphoactivate STAT3 proteins at a specific tyrosine residue (Y705). LanthaScreen® STAT3 GripTite™ is a human cell line that constitutively expresses a GFP-STAT3 fusion protein. The JAK/STAT signaling pathway is known to be functionally intact in this cell line; therefore, the GFP-STAT3 fusion protein serves as a substrate for IL-6-inducible phosphorylation. Using this cell line, a lytic immunoassay has been developed in which the phosphorylation state of GFP-STAT3 is detected in cell lysates using a terbium-labeled anti-pY705-STAT3 antibody in a time-resolved FRET (TR-FRET) readout.

The GFP-STAT3 DNA expression construct was transfected into the GripTite™ HEK 293 cell line using Lipofectamine™ 2000 transfection reagent, and the transfected cells were selected with blasticidin. This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as a sorting marker. The assay utilizing this cell line is validated for EC50 and Z’-factors under optimized conditions using IL-6 as a ligand for JAK-mediated GFP-STAT3 phosphorylation. This assay has also been tested under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance, and assay equilibration time. An alternate "mix-and-read" assay format is also described.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists
IL-6
Antagonists
IFN-alpha
Assay Entry
Cell-based terbium phospho-specific activity (stable)
Cell State
Dividing Cells
Gene Symbol
STAT3
Primary Agonist
IL-6
Product Line
GripTite™, LanthaScreen™
Readout
End Point
Reporter Gene
GFP (EmGFP)
Target Entry
STAT3
System Type
LanthaScreen™
Druggable Target
Kinases

Contents & Storage

LanthaScreen™ STAT3 GripTite™ cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

Figures

Frequently asked questions (FAQs)

Citations & References