EpiJET 5-hmC Enrichment Kit

Catalog number: K1491BID

Thermo Scientific™  Related applications: Methylation Analysis

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The Thermo Scientific EpiJET 5-hmC Enrichment Kit enables the enrichment of 5-hmC containing DNA over unmodified DNA and 5-mC DNA. The kit takes advantage of the 5-hmC Modifying Enzyme, which is formulated for highly specific and efficient modification of 5-hmC present in CpG dinucleotides of DNA and is deficient for any activity over unmodified or methylated cytosines. The enzyme adds a short linker molecule to 5-hmC, to which biotin is conjugated in the following chemical modification step. The resulting modified DNA is separated from unmodified DNA using streptavidin-coated magnetic beads.

More than 50-fold enrichment of the 5-hmC modified DNA over unmodified and 5-mC containing DNA and at least 20% recovery of the input 5-hmC DNA can be achieved. For performance evaluation of the kit, specific DNA controls (unmodified and 5-hmC DNA) are included.

Specific—no activity over unmodified or methylated cytosines
Versatile—efficient enrichment of single and multiple 5-hmC targets
Robust—consistent yield and enrichment
Fast—procedure completed in 3 hours
Pure—enriched DNA suitable for downstream applications

• qPCR
• Sequencing
• Microarrays
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 25 reactions

Contents & storage

Box 1/2 includes:
• 5-hmC Modifying Enzyme
• 4X Enzyme Reaction Buffer
• 10X Biotin Conjugation Buffer
• Biotin Reagent
• Primer Mix for Control DNA (unmodified), 5 µM
• Primer Mix for 5-hmC Control DNA, 5 µM
• 5-hmC Control DNA, 10 ng/µL
• Control DNA (unmodified), 0.5 µg/µL

Box 2/2 includes
• Streptavidin Magnetic Beads
• Streptavidin Binding Buffer
• Streptavidin Wash Buffer 1
• Streptavidin Wash Buffer 2
• Water, nuclease-free
• Column Binding Buffer
• Column Wash Buffer (concentrated)
• Elution Buffer
• DNA Purification Micro Columns and Collection Tubes


Manuals & protocols