The GeneBLAzer® NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat # K1129) contain a beta-lactamase (bla) reporter gene under control of the nuclear factor of activated T-cell (NFAT) response element.
The NPSR1-A CHO-K1 DA cells and NPSR1-A-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using Neuropeptide S. In addition, NPSR1-A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary found in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.
Cell-based beta lactamase reporter gene
Single Cell Clone Origin
Contents & Storage
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: o GeneBLAzer™ NPSR1-A CHO-K1 DA cells o LiveBLAzer™-FRET B/G Loading Kit, 70µg o Solution D o GeneBLAzer™ NPSR1-A CHO-K1 Assay Protocol o LiveBLAzer™-FRET B/G Loading Protocol o Certificate of Analysis The GeneBLAzer™ NPSR1-A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.