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Thermo Scientific™

CellSensor™ SBE-bla A375 Cell Line

Catalog number:  K1514

Related applications:

Pharma & Biopharma

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Description

Bone morphogenetic proteins (BMPs), members of the transforming growth factor beta (TGF-β) superfamily, play important roles in the development of the heart, central nervous system and cartilage. Disruption of BMP signaling affects the body plan of the developing embryo. BMPs propagate their signal by activating Activin receptor-like kinase (ALK), which in turn mediates the phosphorylation of R-Smads. Phosphorylated R-Smad associates with Smad4 and then translocates to the nucleus regulating gene expression. A375 cells are human malignant melanoma cells containing endogenous Smad signaling pathway. SBE-bla A375 cell line was engineered to express beta-lactamase under the control of Smad binding element. This cell line has been validated for Z' and EC50 under optimized conditions using BMP-4. This cell line has also responded to Nodal and was tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, substrate loading time. Additional information using various small molecule inhibitors and StealthTM RNAi is also provided.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: A375
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: Signaling Pathway
Product Line: CellSensor™
System Type: CellSensor™
Target Entry: ALK5, ALK7, TGF beta/BMP pathway

Contents & storage

CellSensor® SBE-bla A375 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.