Tango™ CCR7-bla U2OS cells contain the human Chemokine (C-C motif) receptor 7 (CCR7) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ CCR7-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of MIP-3b. In addition, Tango™ CCR7-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_001838.2
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Native Tail
Detection Method
Fluorescent
Gene Symbol
CCR7
Population Status
Pool of Stable Cells
Product Line
Tango™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
CCR7
Type
U2OS Cell
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs
Contents & Storage
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o Tango™ CCR7-bla U2OS cells o Tango™ CCR7-bla U2OS Assay Protocol o Certificate of Analysis The Tango™ CCR7-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.