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Thermo Scientific™

CellSensor™ CRE-bla HEK 293T Cell Line

Catalog number:  K1540

Related applications:

Pharma & Biopharma

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Description

The CellSensor® CRE-bla HEK 293T cell line contains the b-lactamase reporter gene under control of the cAMP response element (CRE). To obtain the cell line, the CRE-bla construct was transduced into HEK 293T cells by lentivirus. Subsequent flow cytometry was used to isolate clones responsive to forskolin stimulation. The CellSensor® CRE-bla HEK 293T cell line can be used to build specific G-protein coupled receptor (GPCR) assays or to detect changes in intracellular cAMP levels. Academic and non-profit customers, please inquire for special pricing.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Cell-based beta lactamase reporter gene
Cell State: Dividing Cells
Druggable Target: Signaling Pathway
Product Line: CellSensor™
System Type: CellSensor™
Target Entry: PKA, cAMP, cAMP/PKA pathway

Contents & storage

CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.