Invitrogen™
RiboMinus™ Human/Mouse Transcriptome Isolation Kit
Description
The RiboMinus™ Human/Mouse Transcriptome Isolation Kit constitutes a novel purification system that enriches the whole spectrum of mRNA species by depleting large ribosomal RNA molecules in a total RNA sample. The kit removes >95% of 18S and 28S rRNA molecules from human or mouse total RNA (Figure 1) without relying on current polyA+ purification methods. This increases the representation of mRNA species, including non-polyA-tailed, fragmented mRNA or other regulatory RNAs that cannot be purified via polyA-selection methods. Purified RNA is suitable for several downstream applications, including, library construction, RT-PCR, and microarray (Figure 2).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Downstream Application: | Microarray Analysis,Northern Blotting,Real-Time Quantitative PCR (qPCR),Reverse Transcriptase PCR (RT-PCR),cDNA Library Construction |
|---|---|
| Final Product: | Transcriptome RNA |
| Format: | Magnetic Bead |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Isolation Technology: | Magnetic Bead |
| Product Line: | RiboMinus™ |
| Product Size: | 6 preps |
| Sample Type (General): | RNA |
| Sample Type (Specific): | Total RNA |
| System: | RiboMinus |
Contents & storage
The RiboMinus™ Human/Mouse Transcriptome Isolation Kit contains two modules; the RiboMinus™ Human/Mouse Probe Module and the Concentration Module. The RiboMinus™ Human/Mouse Probe Module contains the RiboMinus™ magnetic beads, the RiboMinus™ human/mouse probe, and binding buffer. The Concentration Module contains spin cartridges, wash tubes, recovery tubes, binding buffer, wash buffer, and RNase-free water. Store the RiboMinus" Human/Mouse Probe Module at +4·C. Store the Concentration Module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.
