RiboMinus™ Human/Mouse Transcriptome Isolation Kit

Catalog number: K155001

Ambion™  Related applications: Microarray Analysis | RNA Extraction

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The RiboMinus™ Human/Mouse Transcriptome Isolation Kit constitutes a novel purification system that enriches the whole spectrum of mRNA species by depleting large ribosomal RNA molecules in a total RNA sample. The kit removes >95% of 18S and 28S rRNA molecules from human or mouse total RNA (Figure 1) without relying on current polyA+ purification methods. This increases the representation of mRNA species, including non-polyA-tailed, fragmented mRNA or other regulatory RNAs that cannot be purified via polyA-selection methods. Purified RNA is suitable for several downstream applications, including, library construction, RT-PCR, and microarray (Figure 2).
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): RNA
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Northern Blotting
Format: Magnetic Bead
System: RiboMinus
Product Size: 6 preps
Final Product: Transcriptome RNA
Isolation Technology: Magnetic Bead
Sample Type (Specific): Total RNA

Contents & storage

The RiboMinus™ Human/Mouse Transcriptome Isolation Kit contains two modules; the RiboMinus™ Human/Mouse Probe Module and the Concentration Module. The RiboMinus™ Human/Mouse Probe Module contains the RiboMinus™ magnetic beads, the RiboMinus™ human/mouse probe, and binding buffer. The Concentration Module contains spin cartridges, wash tubes, recovery tubes, binding buffer, wash buffer, and RNase-free water. Store the RiboMinus" Human/Mouse Probe Module at +4·C. Store the Concentration Module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols

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