Invitrogen™

RiboMinus™ Concentration Module

Catalog number:  K155005

 Related applications:

RNA Extraction

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Description

The RiboMinus Concentration Module is used to concentrate RiboMinus RNA while retaining species of <200 nucleotides (nt) for further use in downstream applications.

Generally, RNA species of <200 nt are excluded from silica spin columns under standard binding conditions for RNA isolation. Studies have shown the importance of these small RNA species, which include regulatory RNA molecules such as microRNA (miRNA), short interfering RNA (siRNA), and snRNA. To retain RNA of <200 nt during the concentration step of RiboMinus RNA isolation, the RiboMinus Concentration Module is used with a modified silica spin column protocol (binding of RNA is performed with 60% ethanol).

The binding conditions of the spin column protocol are optimized for the RiboMinus RNA sample with ethanol and buffer. The sample is loaded onto a spin column. The RiboMinus RNA binds to the silica-based membrane in the column and impurities are removed by thorough washing with Wash Buffer. The RiboMinus RNA is then eluted in RNase-free water.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): RNA
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Northern Blotting, Reverse Transcriptase PCR (RT-PCR)
Final Product: Transcriptome RNA
Isolation Technology: Magnetic Bead
Product Line: RiboMinus™
Product Size: 6 preps
Sample Type (Specific): RiboMinus™ RNA
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature.

Documents

Manuals & protocols