PureLink® Total RNA Blood Kit

Catalog number: K156001

Ambion™  Related applications: RNA Extraction

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Description

PureLink® Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 µl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Simple RNA Purification from Whole Blood
PureLink® Total RNA Blood Kit is designed for purifying from 50 to 500 µl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink® Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink® Total RNA Blood Kit.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
Northern Blotting Kits & Reagents
Nuclease protection assays
Microarray
• cDNA synthesis
Real-Time PCR (qPCR)

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Specifications

Product Size: -
Material de la cámara: Type 304, mirror finish, stainless steel
Intervalo de concentración de CO2: 0 to 20%
Tecnología del sensor de CO2: TC
Control de oxígeno: 1 to 20%
Tecnología del sensor de O2: TC
Humedad relativa: Ambient to 95% @ 37°C (98.6°F)
Rango de temperatura (métrico): 5°C above ambient to 55°C
Altura (imperial) exterior: 39.5 in.
Altura (métrico) exterior: 100.3cm
Anchura (imperial) exterior: 26.0 in.
Anchura (métrico) exterior: 66.0cm
Longitud (imperial) exterior: 25.0 in. (F to B)
Longitud (métrico) exterior: 63.5cm (F to B)
Altura (imperial) interior: 26.8 in.
Altura (métrico) interior: 68.1cm
Fondo (imperial) interior: 20.0 in.
Fondo (métrico) interior: 50.8cm
Anchura (imperial) interior: 21.3 in.
Anchura (métrico) interior: 54.1cm
Peso neto (imperial): 265 lb.
Peso neto (métrico): 120.2kg
Peso con embalaje (imperial): 324 lb.
Peso con embalaje (métrico): 147.0kg
Longitud del estante (imperial): 18.5 in.
Longitud del estante (métrico): 47.0cm
Anchura (imperial) del estante: 18.5 in.
Anchura (métrico) del estante: 47.0cm
Voltaje: 230V
Amperaje: 2.0 FLA (Operating range 180–250V)
Salidas de datos: 4–20 milliamp (select one)
Material de la cabina: 18 gauge, cold-rolled steel, powder coated
Control de concentración de CO2: Better than ±0.1%
Hertz: 50/60Hz
Puertos: Fill Port: 3/8 in. hose (barbed)
Drain Port: 1/4 in. hose (barbed)
Access Port: 1.3 in. with removable silicone plug with filter)
CO2 Inlet: 1/4 in. hose (barbed)
N.° de estantes: 4 standard, 17 maximum
Material del estante: Stainless steel, perforated
Certificaciones/conformidad: UL Listed to United States and Canadian requirements and bear the CE Mark
Dimensiones interiores (longitud x anchura x altura): 20.0 x 21.3 x 26.8 in. (50.8 x 54.1 x 68.1cm)
Requisitos eléctricos: 230V 50/60Hz
Dimensiones exteriores (longitud x anchura x altura): 25 x 26 x 39.5 in. (63.5 x 66 x 100.3cm)

Contents & storage

Store at room temperature.

Documents

Manuals & protocols

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