Invitrogen™

mRNA Catcher™ PLUS Purification Kit

Catalog number: K157002
Invitrogen™

mRNA Catcher™ PLUS Purification Kit

Catalog number: K157002
Catalog Number
K157002
also known as K1570-02
Unit Size
96 preps
Price (USD)
Price: 390.00
Your Price:
Availability
***
Quantity
Catalog Number
K157003
also known as K1570-03
Unit Size
960 preps
Price (USD)
Price: 2,995.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K157002
also known as K1570-02
96 preps
Price: 390.00
Your Price:
***
K157003
also known as K1570-03
960 preps
Price: 2,995.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

Robust performance—high binding capacity per well (>80 ng/well)
Reproducible results—low CVs from well-to-well and plate-to-plate (∼2%)
Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 µL blood)
Compatible plate design—may be used with most PCR cyclers

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2%
Format
96-well Plate
Isolation Technology
Oligo dT Affinity
Purification Time
1.5 hr.
Elution Volume
80 µl
Sample Type
Mammalian Cells, Tissue (Mammalian), Total RNA, Whole Blood
Final Product Type
mRNA
High-throughput Compatibility
High-throughput Compatible, Automated Protocols
For Use With (Equipment)
Robotic Liquid Handling Systems
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
No. of Reactions
96 Preps
Product Line
mRNA CATCHER™
Quantity
1
Shipping Condition
Wet Ice
Target
mRNA
System Type
mRNA Catcher

Contents & Storage

• 1 96-well mRNA Catcher™ PLUS plate (4°C)
• 4 ml 2X Lysis Buffer L20 (room temperature)
• 30 ml Wash Buffer W15 (room temperature)
• 4 ml Dilution Buffer DL1 (room temperature)
• 8 ml Elution Buffer E3 (room temperature)
• 100 µl 0.5M DTT (4°C)

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