The Tango™ SSTR5-bla U2OS cells contain the human Somatostatin Receptor 5 (SSTR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ SSTR5-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ SSTR5-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_001053
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Detection Method
Fluorescent
Gene Symbol
SSTR5
Population Status
Single Cell Clone Origin
Product Line
Tango™
Reporter Gene
BLA (Beta-Lactamase)
Shipping Condition
Room Temperature
Target Entry
SSTR5
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs
Contents & Storage
K1575 Tango™ SSTR5-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ SSTR5-bla U2OS cells • Tango™ SSTR5-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ SSTR5-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.