RNA editing generates a diverse array of transcripts encoding squid Kv2 K+ channels with altered functional properties.
AuthorsPatton DE, Silva T, Bezanilla F
JournalNeuron
PubMed ID9331360
We have cloned a Kv2 potassium channel from squid optic lobe termed sqKv2. Multiple overlapping sqKv2 cDNA clones differed from one another at specific positions by purine transitions. To test whether the purine transitions were generated by RNA editing, we compared a 360 nucleotide genomic sequence with corresponding cDNA sequences ... More
Structure and expression of the membrane proteoglycan betaglycan, a component of the TGF-beta receptor system.
AuthorsLopez-Casillas F, Cheifetz S, Doody J, Andres JL, Lane WS, Massague J
JournalCell
PubMed ID1657406
We describe the primary structure of rat betaglycan, a polymorphic membrane-anchored proteoglycan with high affinity for transforming growth factor-beta (TGF-beta). As deduced from its cDNA sequence, the 853 amino acid core protein of betaglycan has an extracellular domain with clustered sites for potential attachment of glycosaminoglycan chains. These chains are ... More
Stereotyped and specific gene expression programs in human innate immune responses to bacteria.
AuthorsBoldrick Jennifer C; Alizadeh Ash A; Diehn Maximilian; Dudoit Sandrine; Liu Chih Long; Belcher Christopher E; Botstein David; Staudt Louis M; Brown Patrick O; Relman David A;
JournalProc Natl Acad Sci U S A
PubMed ID11805339
The innate immune response is crucial for defense against microbial pathogens. To investigate the molecular choreography of this response, we carried out a systematic examination of the gene expression program in human peripheral blood mononuclear cells responding to bacteria and bacterial products. We found a remarkably stereotyped program of gene ... More
HERF1, a novel hematopoiesis-specific RING finger protein, is required for terminal differentiation of erythroid cells.
AuthorsHarada H, Harada Y, O'Brien DP, Rice DS, Naeve CW, Downing JR
JournalMol Cell Biol
PubMed ID10207104
'The AML1/core binding factor beta (CBFbeta) transcription factor is essential for definitive hematopoiesis; however, the downstream pathways through which it functions remain incompletely defined. Using a differential cloning approach to define components of this pathway, we have identified a novel gene designated HERF1 (for hematopoietic RING finger 1), whose expression ... More
Nitric oxide activates diverse signaling pathways to regulate gene expression.
AuthorsHemish J, Nakaya N, Mittal V, Enikolopov G,
JournalJ Biol Chem
PubMed ID12907672
'Nitric oxide signaling is crucial for effecting long lasting changes in cells, including gene expression, cell cycle arrest, apoptosis, and differentiation. We have determined the temporal order of gene activation induced by NO in mammalian cells and have examined the signaling pathways that mediate the action of NO. Using microarrays ... More
Identification of Nogo-66 receptor (NgR) and homologous genes in fish.
AuthorsKlinger M, Taylor JS, Oertle T, Schwab ME, Stuermer CA, Diekmann H,
JournalMol Biol Evol
PubMed ID12949137
'The Nogo-66 receptor NgR has been implicated in the mediation of inhibitory effects of central nervous system (CNS) myelin on axon growth in the adult mammalian CNS. NgR binds to several myelin-associated ligands (Nogo-66, myelin associated glycoprotein, and oligodendrocyte-myelin glycoprotein), which, among other inhibitory proteins, impair axonal regeneration in the ... More
Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B.
AuthorsLi J, Rabinovich BA, Hurren R, Shannon J, Miller RG,
JournalInt Immunol
PubMed ID12618485
'We have characterized the rat NK receptors NKR-P1A and -P1B. A cDNA library was constructed from the rat NK cell line, RNK-16. Using the pMX retroviral cloning system, the library was expressed in the human NK cell line, YTSeco, and cells staining with the anti-rat mAb 10/78 identified, FACS sorted ... More
Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line.
Authors Kekuda R; Prasad P D; Fei Y J; Torres-Zamorano V; Sinha S; Yang-Feng T L; Leibach F H; Ganapathy V;
JournalJ Biol Chem
PubMed ID8702519
'We have isolated a cDNA from a human placental choriocarcinoma cell cDNA library which, when expressed in HeLa cells, induces a Na+-dependent amino acid transport system with preference for zwitterionic amino acids. Anionic amino acids, cationic amino acids, imino acids, and N-methylated amino acids are excluded by this system. These ... More
UNR translation can be driven by an IRES element that is negatively regulated by polypyrimidine tract binding protein.
AuthorsCornelis S, Tinton SA, Schepens B, Bruynooghe Y, Beyaert R,
JournalNucleic Acids Res
PubMed ID15928332
'Upstream of N-ras (Unr) has been described as an internal initiation trans-acting factor (ITAF) in the cap-independent translation of some particular viral and cellular mRNAs. Two factors led us to hypothesize that the UNR 5''-untranslated region (5''-UTR) may contain an internal ribosome entry site (IRES). The first was the requirement ... More
A common mutational pattern in Cockayne syndrome patients from xeroderma pigmentosum group G: implications for a second XPG function.
AuthorsNouspikel T, Lalle P, Leadon SA, Cooper PK, Clarkson SG
JournalProc Natl Acad Sci U S A
PubMed ID9096355
'Xeroderma pigmentosum (XP) patients have defects in nucleotide excision repair (NER), the versatile repair pathway that removes UV-induced damage and other bulky DNA adducts. Patients with Cockayne syndrome (CS), another rare sun-sensitive disorder, are specifically defective in the preferential removal of damage from the transcribed strand of active genes, a ... More
Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.
AuthorsNinomiya-Tsuji J, Nomoto S, Yasuda H, Reed SI, Matsumoto K
JournalProc Natl Acad Sci U S A
PubMed ID1717994
'We have cloned two different human cDNAs that can complement cdc28 mutations of budding yeast Saccharomyces cerevisiae. One corresponds to a gene encoding human p34CDC2 kinase, and the other to a gene (CDK2; cell division kinase) that has not been characterized previously. The CDK2 protein is highly homologous to p34CDC2 ... More
Transcriptional induction of the melanocyte-stimulating hormone receptor in brain metastases of murine K-1735 melanoma.
'Metastatic K-1735 murine melanoma cells are amelanotic in culture or in the subcutis of syngeneic mice. When injected into the internal carotid artery, these cells produce melanotic brain metastases. The production of melanin in tumor cells growing in the brain was directly correlated with induction of melanocyte-stimulating hormone receptor (MSH-R) ... More
Cloning of ELL, a gene that fuses to MLL in a t(11;19)(q23;p13.1) in acute myeloid leukemia.
AuthorsThirman MJ, Levitan DA, Kobayashi H, Simon MC, Rowley JD
JournalProc Natl Acad Sci U S A
PubMed ID7991593
'To characterize the functions of MLL fusion transcripts, we cloned the gene that fuses to MLL in the translocation t(11;19)(q23;p13.1). This translocation is distinct from another type of 11;19 translocation with a 19p13.3 breakpoint that results in the fusion of MLL to the ENL gene. By PCR screening of a ... More
Regulation of sialomucin production in colon carcinoma cells.
'In this study, we investigated the biochemical basis for the induction of sialomucin production in HT-29 LMM human colon carcinoma cells. Previous studies showed that conditioned medium from organ cultures of normal human colonic connective tissues (NCCM) stimulated the biosynthesis of high molecular weight sialoglycoproteins (M(r) 740,000 and 450,000) by ... More
NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus.
AuthorsMasuda ES, Naito Y, Tokumitsu H, Campbell D, Saito F, Hannum C, Arai K, Arai N
JournalMol Cell Biol
PubMed ID7739550
'The nuclear factor of activated T cells (NFAT) regulates cytokine gene expression in T cells through cis-acting elements located in the promoters of cytokine genes. Here, we report the cDNA cloning, chromosomal localization, and initial characterization of a transcription factor related to NFATp and NFATc. The novel molecule, designated NFATx, ... More
IFN-gamma-dependent IL-7 gene regulation in keratinocytes.
AuthorsAriizumi K, Meng Y, Bergstresser PR, Takashima A
JournalJ Immunol
PubMed ID7751645
'IL-7 plays a central role in regulating the growth and differentiation of T cells. We have reported previously that epidermal keratinocytes produce biologically relevant amounts of IL-7, thereby supporting the growth of epidermal gamma delta T cells. In this report, we report that IL-7 gene expression is regulated in keratinocytes ... More
Dystrophin is transcribed in brain from a distant upstream promoter.
AuthorsBoyce FM, Beggs AH, Feener C, Kunkel LM
JournalProc Natl Acad Sci U S A
PubMed ID1996328
'Dystrophin, the protein product of the Duchenne muscular dystrophy gene, is expressed in brain as well as muscle. The role of dystrophin in the brain is not clear, though one-third of Duchenne muscular dystrophy patients exhibit some degree of mental retardation. We have isolated the genomic region encoding the alternative ... More
Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition.
AuthorsStratmann R, Lehner CF
JournalCell
PubMed ID8548823
'Mutations in the Drosophila genes pimples and three rows result in a defect of sister chromatid separation during mitosis. As a consequence, cytokinesis is also defective. However, cell cycle progression including the mitotic degradation of cyclins A and B is not blocked by the failure of sister chromatid separation, and ... More
An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locus.
'The Gnas locus in distal mouse chromosome (Chr) 2 is emerging as a complex genomic region. It contains three imprinted genes in the order Nesp-Gnasxl-Gnas. Gnas encodes a G protein alpha-subunit, and Nesp and Gnasxl encode proteins of unknown function expressed in neuroendocrine tissue. Together, these genes form a single ... More
Identification of a gene, MLL, that spans the breakpoint in 11q23 translocations associated with human leukemias [published erratum appears in Proc Natl Acad Sci U S A 1992 May 1;89(9):4220]
AuthorsZiemin-van der Poel S, McCabe NR, Gill HJ, Espinosa R III, Patel Y, Harden A, Rubinelli P, Smith SD, LeBeau MM, Rowley JD, et al
JournalProc Natl Acad Sci U S A
PubMed ID1720549
'Recurring chromosomal translocations involving chromosome 11, band q23, have been observed in acute lymphoid leukemias and especially in acute myeloid leukemias. We recently showed that breakpoints in four 11q23 translocations, t(4;11)(q21;q23), t(6;11)(q27;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13.3), were contained within a yeast artificial chromosome clone bearing the CD3D and CD3G gene loci. ... More
Simulation of human autosomal dominant retinitis pigmentosa in transgenic mice expressing a mutated murine opsin gene.
AuthorsNaash MI, Hollyfield JG, al-Ubaidi MR, Baehr W
JournalProc Natl Acad Sci U S A
PubMed ID8516292
'Autosomal dominant retinitis pigmentosa (ADRP), slowly progressing over decades, leads to severe visual impairment and in some cases to complete blindness. More than 40 mutations in the human opsin gene have been linked to some forms of this genetically heterogeneous disease. In photoreceptor cells of ADRP patients with mutations in ... More
CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene.
AuthorsRouvier E, Luciani MF, Mattei MG, Denizot F, Golstein P
JournalJ Immunol
PubMed ID8390535
'To detect novel molecules involved in immune functions, a subtracted cDNA library between closely related murine lymphoid cells was prepared using improved technology. Differential screening of this library yielded several clones with a very restricted tissue specificity, including one that we named CTLA-8. CTLA-8 transcripts could be detected only in ... More
A genome-wide functional assay of signal transduction in living mammalian cells.
AuthorsWhitney M, Rockenstein E, Cantin G, Knapp T, Zlokarnik G, Sanders P, Durick K, Craig FF, Negulescu PA
JournalNat Biotechnol
PubMed ID9853613
'We describe a genome-wide functional assay for rapid isolation of cell clones and genetic elements responsive to specific stimuli. A promoterless beta-lactamase reporter gene was transfected into a human T-cell line to generate a living library of reporter-tagged clones. When loaded with a cell-permeable fluorogenic substrate, the cell library simultaneously ... More
The retroviral oncogene qin belongs to the transcription factor family that includes the homeotic gene fork head.
AuthorsLi J, Vogt PK
JournalProc Natl Acad Sci U S A
PubMed ID8099441
Avian sarcoma virus 31 contains an oncogene that we have named qin. qin codes for a nuclear protein, Qin, that is a member of the HNF-3/fork head family of transcriptional regulators. Within this family Qin is particularly closely related to rat brain factor 1 (BF-1), a telencephalon-specific gene presumed to ... More
Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype.
AuthorsWadhwa R, Kaul SC, Ikawa Y, Sugimoto Y
JournalJ Biol Chem
PubMed ID8454632
A novel 66-kDa protein, named p66mot-1, is identified to be associated with cellular mortality by virtue of its presence in cytosolic fractions of serially passaged mouse embryonic fibroblasts (MEF) and the mortal hybrids obtained by the fusion of mortal (MEF) and immortal (MN48-1, derivative of NIH 3T3) cells. Immortal cells ... More
Sodium pump isozymes are differentially expressed in electrically dissimilar regions of colonic circular smooth muscle.
AuthorsBurke EP, Sanders KM, Horowitz B
JournalProc Natl Acad Sci U S A
PubMed ID1848700
Molecular analyses of Na,K-ATPase abundance and alpha-subunit isoform distribution were performed to determine whether pump expression varies at different points through the thickness of the circular layer of colonic smooth muscle. The mRNA and polypeptides of Na,K-ATPase alpha 1 and beta subunits were twice as abundant in the submucosal region ... More
Identification and preliminary characterization of a protein motif related to the zinc finger.
We have identified a protein motif, related to the zinc finger, which defines a newly discovered family of proteins. The motif was found in the sequence of the human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six. We propose naming this motif the RING ... More
Rearrangement of the PAX3 paired box gene in the paediatric solid tumour alveolar rhabdomyosarcoma.
We have determined that PAX3 (found previously to be mutated in Waardenburg syndrome) is the chromosome 2 locus rearranged by the t(2;13)(q35;q14) translocation of the paediatric solid tumour alveolar rhabdomyosarcoma. The rearrangement breakpoints occur within an intron downstream of the paired box and homeodomain-encoding regions. Upstream PAX3 sequences hybridize to ... More
CNS stem cells express a new class of intermediate filament protein.
AuthorsLendahl U, Zimmerman LB, McKay RD
JournalCell
PubMed ID1689217
Multipotential CNS stem cells receive and implement instructions governing differentiation to diverse neuronal and glial fates. Exploration of the mechanisms generating the many cell types of the brain depends crucially on markers identifying the stem cell state. We describe a gene whose expression distinguishes the stem cells from the more ... More
Lymphotactin: a cytokine that represents a new class of chemokine.
AuthorsKelner GS, Kennedy J, Bacon KB, Kleyensteuber S, Largaespada DA, Jenkins NA, Copeland NG, Bazan JF, Moore KW, Schall TJ, et al
JournalScience
PubMed ID7973732
In this study, the cytokine-producing profile of progenitor T cells (pro-T cells) was determined. During screening of a complementary DNA library generated from activated mouse pro-T cells, a cytokine designated lymphotactin was discovered. Lymphotactin is similar to members of both the Cys-Cys and Cys-X-Cys chemokine families but lacks two of ... More
Genetic differences in the ethanol sensitivity of GABAA receptors expressed in Xenopus oocytes.
AuthorsWafford KA, Burnett DM, Dunwiddie TV, Harris RA
JournalScience
PubMed ID1695761
Animal lines selected for differences in drug sensitivity can be used to help determine the molecular basis of drug action. Long-sleep (LS) and short-sleep (SS) mice differ markedly in their genetic sensitivity to ethanol. To investigate the molecular basis for this difference, mRNA from brains of LS and SS mice ... More
Expression of glutamate receptor genes in white matter: developing and adult rat optic nerve.
AuthorsJensen AM, Chiu SY
JournalJ Neurosci
PubMed ID8463842
We have identified the glutamate receptor genes expressed by developing and adult rat optic nerve. Results from PCR suggested that of the ionotropic glutamate receptor (GluR) gene family, only GluR1 and GluR3 subunits are expressed by optic nerve. However, Northern blot analysis demonstrated that only the GluR1 subtype is expressed ... More
Direct amplification of a single dissected chromosomal segment by polymerase chain reaction: a human brain sodium channel gene is on chromosome 2q22-q23.
AuthorsHan JA, Lu CM, Brown GB, Rado TA
JournalProc Natl Acad Sci U S A
PubMed ID1846440
We have devised a general strategy for gene mapping based upon the direct amplification of a target sequence within a single microdissected Giemsa-banded chromosomal segment using the polymerase chain reaction. The usefulness of this approach was demonstrated by mapping a cloned human brain sodium channel (alpha subunit) gene sequence to ... More
Lack of significant differences in association rates and affinities of antibodies from short-term and long-term responses to hen egg lysozyme.
AuthorsGoldbaum FA, Cauerhff A, Velikovsky CA, Llera AS, Riottot MM, Poljak RJ
JournalJ Immunol
PubMed ID10229844
The affinities (Ka) and association rate constants (kon) of 23 mouse (BALB/c) anti-lysozyme mAbs obtained after short and prolonged immunizations have been measured by plasmon resonance techniques. The affinities for the 23 Abs, measured using their Fab, range from Ka = 1.1 x 10(7) to 1.4 x 10(10) M-1. There ... More
Purification, characterization, and cDNA cloning of alpha-N-acetylgalactosamine-specific lectin from starfish, Asterina pectinifera.
AuthorsKakiuchi M, Okino N, Sueyoshi N, Ichinose S, Omori A, Kawabata S, Yamaguchi K, Ito M,
JournalGlycobiology
PubMed ID11886841
We report here the purification, characterization, and cDNA cloning of a novel N-acetylgalactosamine-specific lectin from starfish, Asterina pectinifera. The purified lectin showed 19-kDa, 41-kDa, and 60-kDa protein bands on SDS-PAGE, possibly corresponding to a monomer, homodimer, and homotrimer. Interestingly, on 4-20% native PAGE the lectin showed at least nine protein ... More
Complex functional interactions at the early enhancer of the PQ strain of BK virus.
AuthorsFerguson AT, Subramani S
JournalJ Virol
PubMed ID8207802
BK virus is a human papovavirus that latently infects a majority of the world's population. There are more than 30 strains of the virus, most of which differ in the structure of the early enhancer region. The enhancer of the progenitor strain, WW, from which the other strains can be ... More
Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas.
AuthorsBaron BW, Nucifora G, McCabe N, Espinosa R 3d, Le Beau MM, McKeithan TW
JournalProc Natl Acad Sci U S A
PubMed ID8506375
Chromosomal translocations involving chromosome 3, band q27, are among the most common rearrangements in B-cell non-Hodgkin lymphoma. From a bacteriophage lambda library prepared from a lymphoma characterized by a t(3;14)(q27;q32), genomic clones were isolated using a probe from the immunoglobulin heavy chain locus (IGH) joining region. In addition to clones ... More
Molecular cloning and tissue distribution of keratocan. Bovine corneal keratan sulfate proteoglycan 37A.
Previous studies showed that the keratan sulfate-containing proteoglycans of bovine corneal stroma contain three unique core proteins designated 37A, 37B, and 25 (Funderburgh, J. L., Funderburgh, M. L., Mann, M. M., and Conrad, G. W. (1991) J. Biol. Chem. 266, 14226- 14231). Degenerate oligonucleotides designed from amino acid sequences of ... More
A testis-specific gene encoding a nuclear high-mobility-group box protein located in elongating spermatids.
AuthorsBoissonneault G, Lau YF
JournalMol Cell Biol
PubMed ID8321234
A cDNA encoding a DNA-binding protein has been isolated by screening a mouse testicular expression cDNA library with a concatemer of a 12-bp putative protein-binding element present in the promoter of the testis- specific gene PGK-2. Sequence analysis of the isolated cDNA indicated the presence of an open reading frame ... More
Cloning and characterization of complementary DNA encoding the eukaryotic initiation factor 2-associated 67-kDa protein (p67).
AuthorsWu S, Gupta S, Chatterjee N, Hileman RE, Kinzy TG, Denslow ND, Merrick WC, Chakrabarti D, Osterman JC, Gupta NK
JournalJ Biol Chem
PubMed ID8496145
The eukaryotic initiation factor 2 (eIF-2)-associated 67-kDa glycoprotein (p67) protects eIF-2 alpha-subunit from inhibitory phosphorylation by eIF-2 kinases, such as heme-regulated inhibitor and double-stranded RNA-activated inhibitor. This promotes protein synthesis in the presence of eIF-2 kinases present in animal cells (Ray, M. K., Datta, B., Chakraborty, A., Chattopadhyay, A., Meza- ... More
Expression cloning of a cDNA for human leukotriene C4 synthase, an integral membrane protein conjugating reduced glutathione to leukotriene A4.
AuthorsLam BK, Penrose JF, Freeman GJ, Austen KF
JournalProc Natl Acad Sci U S A
PubMed ID8052639
Leukotriene (LT) C4 synthase, an integral microsomal membrane protein, conjugates LTA4, an epoxide intermediate, to reduced glutathione (GSH) to form a proinflammatory mediator, LTC4. A sensitive fluorescence- linked immunoassay for LTC4 was used to screen a KG-1 cDNA expression library for LTC4 synthase activity after transfection of COS cells and ... More
Otx1 and Otx2 define layers and regions in developing cerebral cortex and cerebellum.
AuthorsFrantz GD, Weimann JM, Levin ME, McConnell SK
JournalJ Neurosci
PubMed ID7931541
Within the cerebral and cerebellar cortices, neurons are organized in layers that segregate neurons with distinctive morphologies and axonal connections, and areas or regions that correspond to distinct functional domains. To explore the molecular underpinnings of pattern formation in layered regions of the CNS, we have characterized the patterns of ... More
An overexpressed gene transcript in senescent and quiescent human fibroblasts encoding a novel protein in the epidermal growth factor- like repeat family stimulates DNA synthesis.
AuthorsLecka-Czernik B, Lumpkin CK Jr, Goldstein S
JournalMol Cell Biol
PubMed ID7799918
We carried out subtractive enrichment of a cDNA library derived from mRNA of senescent human diploid fibroblasts (HDF) established from a subject with Werner syndrome of premature aging. By differential screening, we isolated an overexpressed cDNA sequence (S1-5) that codes for a novel protein containing epidermal growth factor (EGF)-like domains ... More
Alternative promoter usage and splicing options result in the differential expression of mRNAs encoding four isoforms of chicken VBP, a member of the PAR subfamily of bZIP transcription factors.
AuthorsBurch JB, Davis DL
JournalNucleic Acids Res
PubMed ID7984425
We previously isolated a set of overlapping cDNA clones that encoded a unique open reading frame for the chicken VBP transcription factor. We now report the isolation of a cDNA clone that encodes a complete open reading frame for a VBP isoform that differs from the previously reported sequence at ... More
Antimitogenic and mitogenic actions of interleukin-1 in diverse cell types are associated with induction of gro gene expression.
AuthorsRangnekar VV, Waheed S, Davies TJ, Toback FG, Rangnekar VM
JournalJ Biol Chem
PubMed ID1989993
Interleukin-1 (IL-1) is an antiproliferative factor for growing human melanoma A375-C6 cells. To define the molecular basis for the action of IL-1, we set out to identify early genes induced by the cytokine in the absence of de novo protein synthesis. cDNA libraries were constructed from A375-C6 cells that were ... More
The genomic organization of the region containing the Drosophila melanogaster rpL7a (Surf-3) gene differs from those of the mammalian and avian Surfeit loci.
AuthorsArmes N, Fried M
JournalMol Cell Biol
PubMed ID7739520
The Surf-3 gene of the unusually tight mouse Surfeit locus gene cluster has been identified as the highly conserved ribosomal protein gene L7a (rpL7a). The topography and juxtaposition of the Surfeit locus genes are conserved for the 600 million years of divergent evolution between mammals and birds. This suggests cis ... More
Characterization of a 4.8kb transcript from the Duchenne muscular dystrophy locus expressed in Schwannoma cells.
AuthorsBlake DJ, Love DR, Tinsley J, Morris GE, Turley H, Gatter K, Dickson G, Edwards YH, Davies KE
JournalHum Mol Genet
PubMed ID1301145
The 14kb dystrophin transcript from the Duchenne muscular dystrophy (DMD) locus, which encodes a 427kDa protein, is differentially spliced at the amino terminal end giving rise to alternative transcripts expressed in muscle and brain. Here we present evidence for a 4.8kb transcript from the DMD locus which is ubiquitously expressed ... More
A novel transcription factor regulates expression of the vacuolar H+- ATPase B2 subunit through AP-2 sites during monocytic differentiation.
AuthorsLee BS, Krits I, Crane-Zelkovic MK, Gluck SL
JournalJ Biol Chem
PubMed ID8995244
During monocyte-to-macrophage differentiation, the cellular content of vacuolar H+-ATPase (V-ATPase) increases more than 4-fold. We have shown previously that amplified expression of the B2 subunit of the V-ATPase occurs solely by increased transcription, and that the 5'-untranslated region of the B2 gene, containing multiple consensus binding sites for the transcription ... More
Cloning and characterization of chicken YB-1: regulation of expression in the liver.
AuthorsGrant CE, Deeley RG
JournalMol Cell Biol
PubMed ID8321222
A cDNA expression library constructed from day 9 embryonic liver was screened with a previously identified protein binding site in the flanking region of the liver-specific, estrogen-dependent avian apoVLDLII gene. Two of the clones isolated were shown to encode the chicken homolog of the Y-box binding protein, YB-1 (dbpb), which ... More
In situ detection of starvation-induced autophagy.
AuthorsMartinet W, De Meyer GR, Andries L, Herman AG, Kockx MM,
JournalJ Histochem Cytochem
PubMed ID16148314
Autophagy is a regulated bulk degradation process involved in many different human pathologies. Transmission electron microscopy (TEM) is currently the only reliable method for monitoring autophagy in situ. Because TEM is labor intensive, we questioned whether useful marker proteins can be found for unambiguous detection of autophagy in tissue via ... More
Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.
Authors Han J; Flemington C; Houghton A B; Gu Z; Zambetti G P; Lutz R J; Zhu L; Chittenden T;
JournalProc Natl Acad Sci U S A
PubMed ID11572983
BH3-only proteins function at a proximal point in a conserved cell death pathway by binding, through their BH3 domains, to other Bcl-2 family members and triggering mitochondrial events associated with apoptosis. Here, we describe a strongly pro-apoptotic BH3-only protein, designated Bbc3, whose expression increases in response to diverse apoptotic stimuli. ... More
The KH-type RNA-binding protein PSI is required for Drosophila viability, male fertility, and cellular mRNA processing.
Authors Labourier Emmanuel; Blanchette Marco; Feiger Jennie W; Adams Melissa D; Rio Donald C;
JournalGenes Dev
PubMed ID11782446
Direct interactions between RNA-binding proteins and snRNP particles modulate eukaryotic pre-mRNA processing patterns to control gene expression. Here, we report that the conserved U1 snRNP-interacting RNA-binding protein PSI is essential for Drosophila viability. A null PSI mutation is recessive lethal at the first-instar larval stage, and lethality is fully rescued ... More