The GeneBLAzer® PAC1-CRE-bla CHO-K1 cells contain the human Adenylate Cyclase Activating Polypeptide 1 Receptor 1 (ADCYAP1R⁄PAC1) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE). The PAC1-CRE-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of established ligands. In addition, PAC1-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_001118
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene Symbol
ADCYAP1R1
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
ADCYAP1R1, PAC1
Type
Division-Arrested Cells Assay Kit
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCR
Contents & Storage
K1619 GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ PAC1 CHO-K1 DA cells • LiveBLAzer™-FRET B⁄G Loading Kit, 70μg • Solution D • GeneBLAzer™ PAC1 CHO-K1 DA Assay Protocol • LiveBLAzer™-FRET B⁄G Loading Protocol • Certificate of Analysis
Storage: The GeneBLAzer™ PAC1 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.