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Thermo Scientific™

RevertAid First Strand cDNA Synthesis Kit

Catalog number: K1621
Thermo Scientific™

RevertAid First Strand cDNA Synthesis Kit

Catalog number: K1621

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation at temperatures up to 55°C.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail; they can therefore be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Radioactively- and non-radioactively-labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid First Strand cDNA Synthesis Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit with all components necessary for the RT reaction already included

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

 
Catalog Number
K1621
Unit Size
20 reactions
No. of Reactions
20 Reactions
Price (USD)
Price: 120.00
Online price: 98.65
Your price:
Availability
-
Full specifications
Quantity
FormatKit
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseRevertAid
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions20 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 13 kb
Starting MaterialRNA
Unit Size20 reactions
Catalog Number
Unit Size
100 reactions
No. of Reactions
100 Reactions
Price (USD)
Price: 489.00
Online price: 424.65
Your price:
Availability
-
Full specifications
Quantity
FormatKit
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseRevertAid
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions100 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 13 kb
Starting MaterialRNA
Unit Size100 reactions
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeNo. of ReactionsPrice (USD)AvailabilityQuantity
K1621Full specifications
20 reactions20 Reactions
Price: 120.00
Online price: 98.65
Your price:
-
FormatKit
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseRevertAid
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions20 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 13 kb
Starting MaterialRNA
Unit Size20 reactions
K1622Full specifications
100 reactions100 Reactions
Price: 489.00
Online price: 424.65
Your price:
-
FormatKit
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseRevertAid
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Final Product TypeFirst-Strand cDNA
No. of Reactions100 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 13 kb
Starting MaterialRNA
Unit Size100 reactions
Showing 2 of 2

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation at temperatures up to 55°C.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail; they can therefore be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Radioactively- and non-radioactively-labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid First Strand cDNA Synthesis Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit with all components necessary for the RT reaction already included

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

General References:
Verma, I.M., Reverse transcriptase, The Enzymes (Boyer, P.D., ed), Academic Press Inc., vol. 14, 87–103, 1981., Gerard, G.F., D'Alessio, J.M., Methods in Molecular Biology, 16, Humana Press, Totowa, N.J., 73-

Contents & Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.

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