Informational:Inquire about OEM or Commercial Supply version of this product here.
Thermo Scientific™

RevertAid First Strand cDNA Synthesis Kit

Catalog number: K1621
Have Questions?
Thermo Scientific™

RevertAid First Strand cDNA Synthesis Kit

Catalog number: K1621
Have Questions?
Catalog Number
K1621
Unit Size
20 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
100 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K162120 reactions
-
K1622100 reactions
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation at temperatures up to 55°C.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail; they can therefore be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Radioactively- and non-radioactively-labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid First Strand cDNA Synthesis Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit with all components necessary for the RT reaction already included

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Reaction Speed
60 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
42°C
Reverse Transcriptase
RevertAid
Ribonuclease H Activity
Yes
Shipping Condition
Dry Ice
For Use With (Application)
RT-PCR
Final Product Type
First-Strand cDNA
No. of Reactions
20 Reactions
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 13 kb
Starting Material
RNA

Contents & Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text