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The Tango™ EDG6-bla U2OS cells contain the human Endothelial Differentiation, Lysophosphatidic Acid G-protein-coupled Receptor, 6 (EDG6) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ EDG6-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ EDG6-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
For Research Use Only. Not for use in diagnostic procedures.
Contents & storage
LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ EDG6-bla U2OS cells • Tango™ EDG6-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ EDG6-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.