Thermo Scientific™

GeneBLAzer™ CCKAR HEK 293T DA Assay Kit

Catalog number:  K1623

Related applications:

Pharma & Biopharma

Back to top

Description

The GeneBLAzer® CCKAR-NFAT-bla CHO-K1 cells contain the human Cholecystokinin A Receptor (CCKAR) stably integrated into the GeneBLAzer® NFAT-bla CHO-K1 cell line. GeneBLAzer® NFAT-bla CHO-K1 (Cat # K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.

The CCKAR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, CCKAR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_000730.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: 293T (HEK)
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: CCKAR
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: CCKAR

Contents & storage

K1623 GeneBLAzer® CCKAR HEK 293T DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® CCKAR HEK 293T DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• GeneBLAzer® CCKAR HEK 293T DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® CCKAR HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.