Thermo Scientific™

GeneBLAzer™ CCKAR HEK 293T DA Assay Kit

Catalog number: K1623
Thermo Scientific™

GeneBLAzer™ CCKAR HEK 293T DA Assay Kit

Catalog number: K1623
Catalog Number
K1623
Unit Size
8 x 106 cells
Price (USD)
902.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K16238 x 106 cells
902.00
Your Price:
***
Product Overview
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The GeneBLAzer® CCKAR-NFAT-bla CHO-K1 cells contain the human Cholecystokinin A Receptor (CCKAR) stably integrated into the GeneBLAzer® NFAT-bla CHO-K1 cell line. GeneBLAzer® NFAT-bla CHO-K1 (Cat # K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.

The CCKAR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, CCKAR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

Accession Number
NM_000730.2
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene Symbol
CCKAR
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
CCKAR
Cell Line
293T (HEK)
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

K1623 GeneBLAzer™ CCKAR HEK 293T DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ CCKAR HEK 293T DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• GeneBLAzer™ CCKAR HEK 293T DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ CCKAR HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

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