GeneBLAzer® CCKAR HEK 293T DA Assay Kit

Catalog number: K1623

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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The GeneBLAzer® CCKAR-NFAT-bla CHO-K1 cells contain the human Cholecystokinin A Receptor (CCKAR) stably integrated into the GeneBLAzer® NFAT-bla CHO-K1 cell line. GeneBLAzer® NFAT-bla CHO-K1 (Cat # K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.

The CCKAR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, CCKAR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.


System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: 293T (HEK)
Cell State: Division-Arrested Cells
Gene Symbol: CCKAR
Product Size: 8 x 106 cells
Accession Number: NM_000730.2
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Contents & storage

K1623 GeneBLAzer® CCKAR HEK 293T DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
• GeneBLAzer® CCKAR HEK 293T DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• GeneBLAzer® CCKAR HEK 293T DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis

The GeneBLAzer® CCKAR HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.


Manuals & protocols