CCAAT⁄enhancer-binding protein (C⁄EBPa basic leucine zipper transcription factor, is involved in the regulation of mitotic growth arrest and differentiation. Mutations in C⁄EBPare thought to result in diseases such as human acute myeloid leukemia. C⁄EBPis expressed in liver, fat, lung, peripheral leukocytes, epidermis, intestine, and skeletal muscle. C⁄EBPalso plays a role in energy homeostasis, whereas dominant negative mutations cause altered hepatic glucose and glycogen metabolism as well as defects in white adipose tissue differentiation. The CellSensor® C⁄EBP-bla Freestyle™ 293F cell line contains a beta-lactamase reporter gene under control of the C⁄EBP response element stably integrated into Freestyle™ 293F cells. To construct this cell line, the C⁄EBP-bla construct was transduced into Freestyle™ 293F cells by lentivirus. Flow cytometry was used to isolate cells responsive to Phorbol 12-Myristate 13-Acetate (PMA). This cell line was tested under various conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, and functionally validated for Z’ and EC50 concentrations using PMA. Academic and non-profit customers, please inquire for special pricing.