CellSensor™ C/EBP-bla FreeStyle™ 293F Cell Line
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Thermo Scientific™

CellSensor™ C/EBP-bla FreeStyle™ 293F Cell Line

CCAAT/enhancer-binding protein (C/EBPa basic leucine zipper transcription factor, is involved in the regulation of mitotic growth arrest and differentiation. MutationsRead more
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Catalog number K1634
Price (USD)/ Each
10,930.00
-
Add to cart
Price (USD)/ Each
10,930.00
Add to cart
CellSensor™ C/EBP-bla FreeStyle™ 293F Cell Line
Catalog numberK1634
Price (USD)/ Each
10,930.00
-
Add to cart

CCAAT/enhancer-binding protein (C/EBPa basic leucine zipper transcription factor, is involved in the regulation of mitotic growth arrest and differentiation. Mutations in C/EBPare thought to result in diseases such as human acute myeloid leukemia. C/EBPis expressed in liver, fat, lung, peripheral leukocytes, epidermis, intestine, and skeletal muscle. C/EBPalso plays a role in energy homeostasis, whereas dominant negative mutations cause altered hepatic glucose and glycogen metabolism as well as defects in white adipose tissue differentiation. The CellSensor™ C/EBP-bla Freestyle™ 293F cell line contains a beta-lactamase reporter gene under control of the C/EBP response element stably integrated into Freestyle™ 293F cells. To construct this cell line, the C/EBP-bla construct was transduced into Freestyle™ 293F cells by lentivirus. Flow cytometry was used to isolate cells responsive to Phorbol 12-Myristate 13-Acetate (PMA). This cell line was tested under various conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, and functionally validated for Z' and EC50 concentrations using PMA. Academic and non-profit customers, please inquire for special pricing.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay EntryCell-based beta lactamase reporter gene
Cell StateDividing Cells
Product LineCellSensor™
Quantity1 Vial
Target EntryGSK3B, C/EBP pathway, C/EBPa
System TypeCellSensor™
Druggable TargetSignaling Pathway
Unit SizeEach
Contents & Storage
CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.