Informational:Inquire about OEM or Commercial Supply version of this product here.
Informational:Maxima H Minus cDNA Synthesis Master Mix now available. Get a sample »
Thermo Scientific™

Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Catalog number: K1672
Thermo Scientific™

Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Catalog number: K1672

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. It provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. These cDNA Synthesis kits use Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT.

 
Catalog Number
K1672
Unit Size
Each
Includes
Kit with dsDNase
No. of Reactions
200 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
50 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
200 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit with dsDNase
No. of Reactions
50 Reactions
Price (USD)
Availability
-
Full specifications
Quantity
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeIncludesNo. of ReactionsPrice (USD)AvailabilityQuantity
K1672Full specifications
Each Kit with dsDNase200 Reactions
-
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
K1641Full specifications
Each Kit only50 Reactions
-
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
K1642Full specifications
Each Kit only200 Reactions
-
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions200 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
K1671Full specifications
Each Kit with dsDNase50 Reactions
-
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
IncludesKit with dsDNase
Unit SizeEach
Showing 4 of 4

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 μg) at elevated temperatures (42–65°C). The synthesis reaction can be completed in 15–30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase also contains a novel double-strand specific DNase engineered to remove contaminating genomic DNA from RNA preps in two minutes without damage to quality or quantity of RNA. Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Features of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR include:

  • High yields of full length cDNA up to 20 kb
  • Efficient cDNA synthesis at wide temperature range (42–65°C)
  • Increased synthesis rate—complete cDNA synthesis in 15–30 minutes
  • High sensitivity and specificity
  • Integrated gDNA removal step with dsDNase kit

Maxima First Strand cDNA Synthesis Kit with dsDNase contains:

  • Maxima Enzyme Mix
  • 5X Reaction Mix
  • nuclease-free water

Kit with dsDNase also contains

  • dsDNase
  • 10X dsDNase Buffer

Applications

  • 2-step RT-PCR
  • 2-step RT-qPCR

Additional information about reaction components

The Maxima Enzyme Mix contains Maxima Reverse Transcriptase and RiboLock RNase Inhibitor. The recombinant RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B, and C at temperatures up to 55°C.

5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers. Nuclease-free water is provided for reaction set-up and dilution of sample RNA.

The absence of endo- and exo-deoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
• 5X Reaction Mix
• dsDNase
• 10X dsDNase Buffer
• Nuclease-free water

Store at –20°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text