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Thermo Scientific™

CellSensor™ ISRE-bla RA-1 Cell Line

Catalog number:  K1674

Related applications:

Pharma & Biopharma

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Description

The CellSensor® ISRE-bla RA-1 Cell Line contains the beta-lactamase (bla) gene under the control of the interferon-stimulated response element (ISRE). This cell line was engineered by lentiviral transduction of an ISRE-bla construct into RA-1 cells. Flow cytometry was then used to isolate a pool of cells responsive to polyinosinic-polycytidylic acid (poly I:C) stimulation. ISRE-bla RA-1 cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, substrate loading time, as well as validated for Z'-factor and EC50 concentrations of poly I:C. Additional testing data using alternate stimuli are also available. Academic and non-profit customers, please inquire for special pricing.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Cell-based beta lactamase reporter gene
Cell State: Dividing Cells
Druggable Target: Cytokines, Signaling Pathway
Product Line: CellSensor™
System Type: CellSensor™
Target Entry: TLR3/TBK1/IRF3 pathway, TBK1, IRF3, TLR3

Contents & storage

CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.