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      GeneBLAzer™ ERα-UAS-bla GripTite™ Cells

      Catalog number:  K1688

       Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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      Description

      ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla GripTite™ cell line. GeneBLAzer®UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Alternate Agonists: Betamethasone, Budesonide, Cortisol
      Antagonists: Cortisone, Milfepristone, Progesterone
      Cell State: Dividing Cells
      Detection Method: Fluorescent
      Druggable Target: Nuclear Receptors
      Primary Agonist: Dexamethasone
      Product Line: GeneBLAzer®
      Product Size: 2 x 106 cells
      Reporter: BLA (Beta-Lactamase)
      System: GeneBLAzer™
      Shipping Condition: Room Temperature

      Contents & storage

      Shipping: Dry Ice Storage: -80°C/Liquid Nitrogen Contains: ER alpha-UAS-bla GriptTite™ cells (K1090)

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