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ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla GripTite™ cell line. GeneBLAzer®UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Alternate Agonists
Betamethasone, Cortisol, Budesonide
Antagonists
Milfepristone, Cortisone, Progesterone
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
Druggable Target
Nuclear Receptors
Primary Agonist
Dexamethasone
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Shipping Condition
Room Temperature
System Type
GeneBLAzer™
Target Entry
ER alpha, ESR1, ERA, NR3A1
Type
GripTite Cells
Contents & Storage
Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains: ER alpha-UAS-bla GriptTite™ cells (K1090)
