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Thermo Scientific™

RevertAid RT Reverse Transcription Kit

Catalog number: K1691
Have Questions?
Thermo Scientific™

RevertAid RT Reverse Transcription Kit

Catalog number: K1691
Have Questions?
Catalog Number
K1691
Unit Size
500 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K1691500 reactions
-
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The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.

First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactions

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Reaction Speed
60 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
42°C
Reverse Transcriptase
RevertAid
Ribonuclease H Activity
Yes
Shipping Condition
Dry Ice
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
No. of Reactions
500 Reactions
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 13 kb
Starting Material
RNA

Contents & Storage

• RevertAid Reverse Transcriptase
• Ribolock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Random Hexamer Primer
• Nuclease-free water

Store at –20°C.


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