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Thermo Scientific™

GeneBLAzer™ RXRβ-UAS-bla HEK 293T Cells

Catalog number:  K1694

Related applications:

Pharma & Biopharma

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Description

RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. RXR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of 9-cis retinoic acid (9-cis-RA). In addition, RXR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists: Progesterone
Antagonists: RU 486
Assay Entry: Cell-based beta lactamase reporter gene
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Primary Agonist: R5020
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: RXR beta, NR2B2, RXRB

Contents & storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains: RXR beta-UAS-bla HEK 293T cells (K1259)