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Thermo Scientific™

GeneBLAzer™ VPAC1-CRE-bla CHO-K1 Cells

Catalog number:  K1705

Related applications:

Pharma & Biopharma

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Description

GeneBLAzer™ VPAC1-CRE-bla CHO-K1 cells contain the human Vasoactive intestinal peptide/pituitary adenylate cyclase activating polypeptide receptor 1 (VPAC1) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer™ VPAC1-CRE-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of VIP. In addition, GeneBLAzer™ VPAC1-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

VPAC1 has been implicated in a wide range of disorders, including Parkinson's disease, septic shock, rheumatoid arthritis, multiple sclerosis, Crohn's disease, and autoimmune diabetes. VPAC1-mediated signaling has also been shown to play a role in HIV infection.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_004624
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: VPAC1
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: VPAC1
Technique: FRET

Contents & storage

Contents:
K1705 GeneBLAzer™ VPAC1-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ VPAC1-CRE-bla CHO-K1 cells
• GeneBLAzer™ VPAC1-CRE-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ VPAC1-CRE-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.