Invitrogen™

Pichia Expression Kit, original kit

Catalog number:  K171001

Related applications:

Protein Biology

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Description

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: P. pastoris
Vector: pPIC
Promoter: AOX1
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Bacterial or Yeast Strain: GS115, KM71
Cloning Method: Restriction Enzyme ⁄ MCS
Expression System: Yeast
Expression Mechanism: Cell-Based Expression
Protein Tag: Untagged

Contents & storage

The Original Pichia Expression Kit includes 10 µg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 µg each 5´ AOX1, 3´ AOX1, and α-factor sequencing primers. Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.